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The other important part of this experimental approach is the concept of control experiments , also known by a shorter term as just controls . A control experiment is a setup where the variable is not introduced, so that it can be directly compared to the experimental situation where the variable (access of dust particles to the broth) is included. So a control experiment for Pasteur's incubation of broth in an open-necked flask would be incubation of broth in the S-necked flasks. If the variable is introduced by tilting the flasks, the control would again be the S-necked flasks. All other conditions (heating temperature, amount of broth, size of flasks, etc.) were the same in the experimental and control situations. The only thing that was different was a single variable  (access of dust particles to the meat broth), because that was the hypothesized source of the living cells that grow in meat broth left out in the open. A single control experiment is usually all that is needed if there is only a single experimental variable being manipulated.

But it is not always possible to simplify a system so that there is only one variable. In those cases, as you will learn in the studio exercises for this class, you might need multiple control experiments. Experiments and controls will also be repeated before the investigator reports the results. It will be described in a way such that other investigators can readily repeat it as well. In some situations the results will be subjected to statistical analysis, although this was not necessary in Pasteur’s experiment. Statistical analysis is critical in many scientific approaches, particularly in studies involving hypotheses about human subjects (e.g., the hypothesis that smoking causes lung cancer), where experimental manipulation of the subjects is difficult or impossible. A scientific experiment, no matter how the results are analyzed, should lead to a conclusion that either supports, or fails to support, the hypothesis. Finally, the experimental results should lead to additional hypotheses, and additional predictions, that can generate further support (or lack of support) for the hypothesis. Try to think of a few additional experiments that you might have suggested to Louis Pasteur if you were alive in 1860, and if you could speak French!

Other aspects of science

The characteristics inherent in the scientific process lead to another property of science, and that is that science is self-correcting . By that we mean that errors can be made, but that continued application of the tools and processes of science will usually lead to elimination of the errors and a more accurate understanding of the natural world. Science never really and finally proves anything to be true; it can, however, prove things to be untrue. To some people, in fact, that characteristic of science, its fluid and changing nature, is maddening. If you require solid ground to stand on, and immutable truth in all aspects of your life, you probably shouldn’t become a scientist. If you find excitement in being part of an enterprise that is constantly changing the extent, and even the nature, of knowledge, then you have some of what it takes to be a scientist. But even if you don’t become a scientist, a bit on scientific knowledge, and a bit of practice with the scientific process, will help you understand the things you need to understand in order to make intelligent decisions about many things, such as health care, climate change, or other concepts that are in your future.

Questions & Answers

what is microbiology
Agebe Reply
What is a cell
Odelana Reply
what is cell
Mohammed
how does Neisseria cause meningitis
Nyibol Reply
what is microbiologist
Muhammad Reply
what is errata
Muhammad
is the branch of biology that deals with the study of microorganisms.
Ntefuni Reply
What is microbiology
Mercy Reply
studies of microbes
Louisiaste
when we takee the specimen which lumbar,spin,
Ziyad Reply
How bacteria create energy to survive?
Muhamad Reply
Bacteria doesn't produce energy they are dependent upon their substrate in case of lack of nutrients they are able to make spores which helps them to sustain in harsh environments
_Adnan
But not all bacteria make spores, l mean Eukaryotic cells have Mitochondria which acts as powerhouse for them, since bacteria don't have it, what is the substitution for it?
Muhamad
they make spores
Louisiaste
what is sporadic nd endemic, epidemic
Aminu Reply
the significance of food webs for disease transmission
Abreham
food webs brings about an infection as an individual depends on number of diseased foods or carriers dully.
Mark
explain assimilatory nitrate reduction
Esinniobiwa Reply
Assimilatory nitrate reduction is a process that occurs in some microorganisms, such as bacteria and archaea, in which nitrate (NO3-) is reduced to nitrite (NO2-), and then further reduced to ammonia (NH3).
Elkana
This process is called assimilatory nitrate reduction because the nitrogen that is produced is incorporated in the cells of microorganisms where it can be used in the synthesis of amino acids and other nitrogen products
Elkana
Examples of thermophilic organisms
Shu Reply
Give Examples of thermophilic organisms
Shu
advantages of normal Flora to the host
Micheal Reply
Prevent foreign microbes to the host
Abubakar
they provide healthier benefits to their hosts
ayesha
They are friends to host only when Host immune system is strong and become enemies when the host immune system is weakened . very bad relationship!
Mark
what is cell
faisal Reply
cell is the smallest unit of life
Fauziya
cell is the smallest unit of life
Akanni
ok
Innocent
cell is the structural and functional unit of life
Hasan
is the fundamental units of Life
Musa
what are emergency diseases
Micheal Reply
There are nothing like emergency disease but there are some common medical emergency which can occur simultaneously like Bleeding,heart attack,Breathing difficulties,severe pain heart stock.Hope you will get my point .Have a nice day ❣️
_Adnan
define infection ,prevention and control
Innocent
I think infection prevention and control is the avoidance of all things we do that gives out break of infections and promotion of health practices that promote life
Lubega
Heyy Lubega hussein where are u from?
_Adnan
en français
Adama
which site have a normal flora
ESTHER Reply
Many sites of the body have it Skin Nasal cavity Oral cavity Gastro intestinal tract
Safaa
skin
Asiina
skin,Oral,Nasal,GIt
Sadik
How can Commensal can Bacteria change into pathogen?
Sadik
How can Commensal Bacteria change into pathogen?
Sadik
all
Tesfaye
by fussion
Asiina
what are the advantages of normal Flora to the host
Micheal
what are the ways of control and prevention of nosocomial infection in the hospital
Micheal
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Source:  OpenStax, Principles of biology. OpenStax CNX. Aug 09, 2016 Download for free at http://legacy.cnx.org/content/col11569/1.25
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