Cells are often ground up with abrasive Madison Christian Microbiology

Question 54 / 97: Cells are often ground up with abrasive to get to their internal enzymes/molecules, followed by removal of the abrasives. Here are two situations and two outcomes. What is the correct interpretation of the results? In situation #1, radioactive amino acids are mixed with ground-up cell material. Radioactive proteins are produced. In situation #2, radioactive amino acids AND the enzyme RNAse (an enzyme that degrades RNA) are mixed with ground-up cell material. No radioactive proteins are produced.

A The mRNA from the cell can be used to make proteins with the radioactive amino acids in the
first situation. In the second situation, the mRNA is destroyed by the RNAse before it can
be translated into protein containing the radioactive amino acids.

B The DNA from the cell can be translated into protein using the radioactive amino acids in the
first situation. The RNAse in the second situation degrades the ribosomal RNA (rRNA), preventing
ribosomes from forming and making proteins with the radioactive amino acids.

C The radioactivity in the amino acids corrupts the tRNA molecules, leading to no protein production
in the second scenario.

D The results cannot be interpreted-there isn't enough information given in the question.

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Microbiology chapters 5-8 Midterm Practice

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