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By the end of this section, you will be able to:
  • Describe the diffraction limit on resolution
  • Describe the diffraction limit on beam propagation

Light diffracts as it moves through space, bending around obstacles, interfering constructively and destructively. This can be used as a spectroscopic tool—a diffraction grating disperses light according to wavelength, for example, and is used to produce spectra—but diffraction also limits the detail we can obtain in images.

[link] (a) shows the effect of passing light through a small circular aperture . Instead of a bright spot with sharp edges, we obtain a spot with a fuzzy edge surrounded by circles of light. This pattern is caused by diffraction, similar to that produced by a single slit. Light from different parts of the circular aperture interferes constructively and destructively. The effect is most noticeable when the aperture is small, but the effect is there for large apertures as well.

Figure a shows a bright white circle on a black background. Its edge is diffused. Figures b and c show two white circles overlapping. The circles in figure c are closer to each other than those in figure b.
(a) Monochromatic light passed through a small circular aperture produces this diffraction pattern. (b) Two point-light sources that are close to one another produce overlapping images because of diffraction. (c) If the sources are closer together, they cannot be distinguished or resolved.

How does diffraction affect the detail that can be observed when light passes through an aperture? [link] (b) shows the diffraction pattern produced by two point-light sources that are close to one another. The pattern is similar to that for a single point source, and it is still possible to tell that there are two light sources rather than one. If they are closer together, as in [link] (c), we cannot distinguish them, thus limiting the detail or resolution    we can obtain. This limit is an inescapable consequence of the wave nature of light.

Diffraction limits the resolution in many situations. The acuity of our vision is limited because light passes through the pupil, which is the circular aperture of the eye. Be aware that the diffraction-like spreading of light is due to the limited diameter of a light beam, not the interaction with an aperture. Thus, light passing through a lens with a diameter D shows this effect and spreads, blurring the image, just as light passing through an aperture of diameter D does. Thus, diffraction limits the resolution of any system having a lens or mirror. Telescopes are also limited by diffraction, because of the finite diameter D of the primary mirror.

Just what is the limit? To answer that question, consider the diffraction pattern for a circular aperture, which has a central maximum that is wider and brighter than the maxima surrounding it (similar to a slit) ( [link] (a)). It can be shown that, for a circular aperture of diameter D , the first minimum in the diffraction pattern occurs at θ = 1.22 λ / D (providing the aperture is large compared with the wavelength of light, which is the case for most optical instruments). The accepted criterion for determining the diffraction limit    to resolution based on this angle is known as the Rayleigh criterion    , which was developed by Lord Rayleigh in the nineteenth century.

Questions & Answers

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studies of microbes
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Assimilatory nitrate reduction is a process that occurs in some microorganisms, such as bacteria and archaea, in which nitrate (NO3-) is reduced to nitrite (NO2-), and then further reduced to ammonia (NH3).
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This process is called assimilatory nitrate reduction because the nitrogen that is produced is incorporated in the cells of microorganisms where it can be used in the synthesis of amino acids and other nitrogen products
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Prevent foreign microbes to the host
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they provide healthier benefits to their hosts
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They are friends to host only when Host immune system is strong and become enemies when the host immune system is weakened . very bad relationship!
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cell is the smallest unit of life
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cell is the structural and functional unit of life
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is the fundamental units of Life
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There are nothing like emergency disease but there are some common medical emergency which can occur simultaneously like Bleeding,heart attack,Breathing difficulties,severe pain heart stock.Hope you will get my point .Have a nice day ❣️
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Source:  OpenStax, University physics volume 3. OpenStax CNX. Nov 04, 2016 Download for free at http://cnx.org/content/col12067/1.4
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