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We encountered many limitations throughout this project. We obtained a small data set consisting of only bright field images. In total only 30 images were used per cell type, which had to be further divided into training, validation, and test images. Secondly, our low image resolution and poor consistency of image contrast affected segmentation, feature extraction, and classification. We were also unable to segment overlapping cells due to difficulty applying thresholding. Finally, all monocytes were misclassified in our classification methods.
Beside improving the current system, adding more automatic features would be desired. The present system still requires manual cropping and resizing of the images before segmentation. The future segmentation should be able to isolate sub-images of single WBC from a multi-WBC background.
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