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- Molecular biology of the gene
- Molecular biology of the gene
- Multiple choice questions
- 1. One can distinguish prokaryotic chromosomes from eukaryotic chromosomes by determining:
- a. Nucleotide sequence
- b. Chromosome-linked proteins
- c. Base composition
- d. Secondary structure
- 2. In E. coli DNA replication, primer is:
- a. A deoxyribonucleotide short sequence
- b. A short RNA annealing to the 3’ end of the template strand
- c. A short RNA complementary to the 5’ end of the leading strand
- d. Synthesized by DNA polymerase I
- 3. Shine-Dalgarno sequence is:
- a. Found at the 3’ end of a prokaryotic gene
- b. Found in 16S rRNA
- c. Complementary to an mRNA sequence
- d. Located upstream of the AUG initiation codon of a prokaryotic mRNA
- 4. If the uracil content is exhausted, the following process will immediately stop:
- a. Reverse transcription
- b. Transcription
- c. Replication
- d. Translation
- 5. The promoter is:
- a. A factor involving in translational process
- b. Associated with repressor in an inducible operon
- c. A sequence located at the 3’ end of a gene
- d. The binding site for RNA polymerase
- 6. Proofreading activity of DNA polymerase III relies on:
- a. The Mut S, H, L repair system recognizing parental DNA methylation
- b. 3’-5’ exonuclease function of DNA polymerase
- c. RNAse H activity
- d. The UvrABC repair system
- 7. The difference on the regulation of gene expression in prokaryotes and eukaryotes is partly due to:
- a. Different environmental conditions
- b. Different cell components
- c. Different cell structural features
- d. Different cell numbers
- 8. The enzyme catalyzing the binding of Alanine to its tRNA is called:
- a. Alanine-tRNA polymerase
- b. Alanine-tRNA transferase
- c. tRNA-Alanyl polymerase
- d. Alanyl-tRNA synthetase
- 9. Microarray analysis can be used to:
- a. Determine the intron-exon organization of a gene
- b. Determine the concentration of a protein in a cell
- c. Determine the stage-specific expression of a gene
- d. Determine the presence of a DNA sequence in a cell
- 10. Hyperchromicity (increased OD value) results from:
- a. Increased light absorbance by double-stranded DNA when it is denatured
- b. Increased light absorbance by double-stranded DNA when it is hydrolyzed
- c. Increased light absorbance by double-stranded DNA contaminated by RNA
- d. Increased light absorbance by double-stranded DNA when it is renatured
- 11. The repair system acting just after the replication finishes is based on:
- a. The elimination of methylated bases
- b. The activities of Methylases
- c. The recognition of hemimethylated DNA strands to be repaired
- d. The excision of the oligonucleotide bearing the mismatch
- 12. The control of gene expression through an operon aims at:
- a. Regulating different gene networks depending on the external stimuli
- b. Regulating stepwise expression of a gene
- c. Exerting a synchronous and fast regulation of genes belonging to one metabolism process
- d. Producing different concentration of proteins of a metabolism process
- 13. Muscle, skin, liver cells differ from each other due to:
- a. Different mutations arisen in each cell type
- b. Different expression of genes in each cell type
- c. Different genes present in different cell types
- d. Different location of cell types in the organism
- 14. Automatic sequencing is based on:
- a. The utilisation of fluorescent labeling
- b. The utilisation of four types of dideoxynucleotide
- c. The utilisation of DNA polymerases
- d. All of the above items
- 15. Which of the following processes is involved in DNA repair:
- a. Conjugation
- b. Reversion of mutation
- c. Transposition
- d. Homologous recombination
- 16. Which of the following processes is characteristic to eukaryotic gene expression control:
- a. Alternative splicing
- b. Alternative use of σ factor
- c. Transcription initiation
- d. Catabolite repression
Source:
OpenStax, Molecular biology of the gene. OpenStax CNX. Jul 29, 2009 Download for free at http://cnx.org/content/col10799/1.1
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