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Card 39 / 79:
HYBRIDIZATION
A genetics lab technique used to identify which colonies of bacteria on a plate contain a particular sequence of DNA or a particular gene . The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that each colony contributes a small smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labeled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the plate. (from BioTech Dictionary)
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